GOanna is used to find annotations for proteins using a similarity search. The input can be a list of IDs or it can be a list of sequences in FASTA format. If the input list is proteins, the user should select blastp as the BLAST program. If the input list is nucleotides, the user should select blastx as the BLAST program. If IDs are used for inputs, the sequences must be available in the AgBase database. GOanna will retrieve the sequences if necessary and conduct the specified BLAST search against a user-specified database. The resulting file contains GO annotations of the top BLAST hits. The sequence alignments are also provided so the user can use these to access the quality of the match.
The BLAST searches conducted by GOanna are time consuming. You will be notified by email when your GOanna results are ready and will be given a web address where they are available for a limited amount of time.